Fig. 2

The impact of As on S. aureus ClfB expression and MAC-T cells inflammatory response. A describes the experimental design (1-exposure of S. aureus to MAC-T cells; 2-adhesion/infection of MAC-T cells with S. aureus to; 3-As inclusion during infection and inhibition of ClfB; 4-MAC-T cells pro-inflammatory response). B shows the impact of As on IL6, IL1β, IL8 and TNFα expression in infected MAC-T cells. C indicates the effect of As on ClfB expression in S. aureus without the presence of MAC-T cells; the expression ClfB in S-aureus co-cocultured with MAC-T cells and As is presented in panel D, E indicates the expression of ClfB in S. aureus MAC-T cells attached or internalised after 6 h of exposure to 0.1, 0.2, 0.5 and 1% As. All experiments were performed in triplicate and the results are represented as means ± SD. Student’s t test was performed to assess significance with the P values being indicated on graphs. The ClfB gene expression is presented as fold change over the infected and As unexposed control. Panel A was created using Biorender